https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Expression of ciliated bronchial epithelium 1 during human spermatogenesis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:31426 CBE1 expression in normal as well as impaired spermatogenesis on mRNA (quantitative reverse-transcription polymerase chain reaction and in situ hybridization) and protein level (immunohistochemistry, Western blot analysis). Result(s): In normal spermatogenesis, CBE1 mRNA was expressed in late pachytene spermatocytes, and the protein was localized within the flagellum of elongating spermatids from stage V up to the spermiation in stage II. Immunoelectron microscopy showed CBE1 clearly associated with microtubules at the manchette, the head-tail coupling apparatus, and the flagellum, but the protein was absent in spermatozoa. Compared with normal spermatogenesis, CBE1 mRNA was statistically significantly reduced in samples with a maturation arrest at the level of round spermatids and primary spermatocytes, and was absent in samples showing Sertoli cell-only syndrome. CBE1 protein was completely missing in SDA samples showing few elongating spermatids. Conclusion(s): Our data strongly suggest an influence of CBE1 in ciliogenesis in spermatids due to the localization at the microtubules of the elongating spermatids, indicating a role in the intramanchette and/or intraflagellar transport mechanism. The absence of CBE1 in spermatozoa suggests that CBE1 is important for the spermatid development but not for the maintenance of mature spermatozoa as a component of the flagellum.]]> Wed 11 Jul 2018 17:04:16 AEST ]]> Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14941 Wed 11 Apr 2018 17:19:33 AEST ]]> DNA double strand breaks but not interstrand crosslinks prevent progress through meiosis in fully grown mouse oocytes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15099 Wed 11 Apr 2018 14:49:33 AEST ]]> RAB-Like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15139 Wed 11 Apr 2018 14:39:08 AEST ]]> RBM5 is a male germ cell splicing factor and is required for spermatid differentiation and male fertility https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14944 Wed 11 Apr 2018 14:30:16 AEST ]]> Identification of the molecular chaperone, heat shock protein 1 (chaperonin 10), in the reproductive tract and in capacitating spermatozoa in the male mouse https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4506 Wed 11 Apr 2018 10:11:11 AEST ]]> Proteomic and functional analysis of human sperm detergent resistant membranes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:13040 Sat 24 Mar 2018 08:16:38 AEDT ]]> Glioma pathogenesis-related 1-Like 1 is testis enriched, dynamically modified, and redistributed during male germ cell maturation and has a potential role in sperm-oocyte binding https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11115 Sat 24 Mar 2018 08:12:26 AEDT ]]> Involvement of multimeric protein complexes in mediating the capacitation-dependent binding of human spermatozoa to homologous zonae pellucidae https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17664 Sat 24 Mar 2018 07:57:48 AEDT ]]> Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human spermoocyte recognition https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20323 Sat 24 Mar 2018 07:55:12 AEDT ]]> A novel germ cell protein, SPIF (sperm PKA interacting factor), is essential for the formation of a PKA/TCP11 complex that undergoes conformational and phosphorylation changes upon capacitation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26537 95% of sperm. Anapparent molecular rearrangement in the complex occurs, bringing PRKACA and TCP11 into proximity. Taken together, these results suggest a role for the novel complex of SPIF, PRKACA, and TCP11 during spermcapacitation, fertilization, and embryogenesis.]]> Sat 24 Mar 2018 07:23:27 AEDT ]]> GLIPR1L1 is an IZUMO-binding protein required for optimal fertilization in the mouse https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36447 Mon 04 May 2020 16:14:04 AEST ]]> Current global status of male reproductive health https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:55389 Fri 24 May 2024 10:34:53 AEST ]]>