https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:31426 CBE1 expression in normal as well as impaired spermatogenesis on mRNA (quantitative reverse-transcription polymerase chain reaction and in situ hybridization) and protein level (immunohistochemistry, Western blot analysis). Result(s): In normal spermatogenesis, CBE1 mRNA was expressed in late pachytene spermatocytes, and the protein was localized within the flagellum of elongating spermatids from stage V up to the spermiation in stage II. Immunoelectron microscopy showed CBE1 clearly associated with microtubules at the manchette, the head-tail coupling apparatus, and the flagellum, but the protein was absent in spermatozoa. Compared with normal spermatogenesis, CBE1 mRNA was statistically significantly reduced in samples with a maturation arrest at the level of round spermatids and primary spermatocytes, and was absent in samples showing Sertoli cell-only syndrome. CBE1 protein was completely missing in SDA samples showing few elongating spermatids. Conclusion(s): Our data strongly suggest an influence of CBE1 in ciliogenesis in spermatids due to the localization at the microtubules of the elongating spermatids, indicating a role in the intramanchette and/or intraflagellar transport mechanism. The absence of CBE1 in spermatozoa suggests that CBE1 is important for the spermatid development but not for the maintenance of mature spermatozoa as a component of the flagellum.]]> Wed 11 Jul 2018 17:04:16 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14941 Wed 11 Apr 2018 17:19:33 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15099 Wed 11 Apr 2018 14:49:33 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15139 Wed 11 Apr 2018 14:39:08 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14944 Wed 11 Apr 2018 14:30:16 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4506 Wed 11 Apr 2018 10:11:11 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:13040 Sat 24 Mar 2018 08:16:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11115 Sat 24 Mar 2018 08:12:26 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17664 Sat 24 Mar 2018 07:57:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20323 Sat 24 Mar 2018 07:55:12 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26537 95% of sperm. Anapparent molecular rearrangement in the complex occurs, bringing PRKACA and TCP11 into proximity. Taken together, these results suggest a role for the novel complex of SPIF, PRKACA, and TCP11 during spermcapacitation, fertilization, and embryogenesis.]]> Sat 24 Mar 2018 07:23:27 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36447 Mon 04 May 2020 16:14:04 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:55389 Fri 24 May 2024 10:34:53 AEST ]]>